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Name: Leung Kin Man University number: 2009109720 Date of experiment: 25-2-2010 BIOL1133: biologic Science Laboratory Course Experiment 4: Expression of worlds secretin sensory receptor concretion protein in E coli. Aim: The suggest of the experiment is to express human secretin receptor fusion protein in E coli by conducting SDS-PAGE gel cataphoresis and estimate the molecular size of that protein. Abstract: In this experiment, the human secretin receptor fusion protein was expressed exploitation the SDS-PAGE technique. The pre-induced and the sample aft(prenominal) IPTG induction was use to extract the human secretin receptor protein by utilise denaturing buffer zone and elution buffer. The molecular weight of the protein trick also be estimated by comparing the bands on the protein ladder. origin: Massive production of active protein is usually done by recombinant deoxyribonucleic acid technology. E coli. was employ as vector for debut and expr ession of fusion protein. E coli. is a germ-negative bacteria with two-baser membrane layer and gamin plasmid. In this experiment, the N-terminal extracellular theatre of the human secretin receptor in E coli. was expressed using IPTG inducible system.

Secretin is the key hormone that prudent for the regulation of gastrointestinal component part by regulating the secretion of bicarbonate and enzyme from the exocrine pancreas. This send away help to neutralize stomachic acid (hydrochloric acid) and hence prevent an optimum pH care for for intestinal digestion. The action of secretin is in general controlled by high-affinity membrane receptors distribu! ted in our body and it is tissue-specific. The N-terminal tag area of the extracellular domain of the receptor protein is obtained by conducting polymerase chain answer (PCR) mutagenesis method. This technique was achieved by using specific desoxyribonucleic acid primers and DNA strand as a template to start the PCR reaction. The coding sequence was introduced into the bacterial DNA as a mail carrier of the coding sequence. Upon IPTG...If you want to posture a full essay, mold it on our website: BestEssayCheap.com

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